Reaction kineticsCollision theory and activation energy. Order and molecularity of reactions. Kinetics law for zero-order and first-order reactions. Activation energy. Arrhenius equation. EnzymesClassification of enzymes. Thermodynamic and structural aspects of enzymatic catalysis. General acidic and basic catalysis, electrostatic and covalent catalysis, proximity catalysis. Reactions with more than one substrates. Effect of pH and temperature on enzymatic activity. Methods for the dosage of enzyme activity: unit of measurement and specific activity. Methods for enzyme purification. Enzyme kineticsHypothesis of steady state. Michaelis-Menten's equation. Meaning of Km and Vmax. Data interpolation methods to derive the kinetic parameters. Number of turnover. Catalytic efficiency. Enzyme inhibitionReversible and irreversible enzyme inhibitors. Cooperativity and enzyme activity regulation. Allosteric enzymes. Enzymes as reagentsBiocatalysis and industrial enzymology. Immobilization and stabilization techniques. Industrial applications of enzymes as biocatalysts. Biosensors, miniaturized analytical systems.