CYTOLOGY AND HISTOLOGY WITH LABORATORY
The course aims to provide students with the basic knowledge on the morpho-functional organization of cells and animal tissues. The aim of the course is to guide students to understand the characteristics of the morphology and functions of the different cellular organelles, of cell differentiation and specialization and of intercellular and extracellular cell-matrix interactions, the architecture and function of the different tissues. The course includes a practical part of laboratory activities dedicated to the preparation and examination of histological preparations.
Chemical composition of protoplasm: 2 hours
The levels of organization in biology: 2 hours
The main techniques and devices for the study of cells and tissues: 4 hours
The plasma membrane: 8 hours
- structure and functions transport, transport mechanisms), glycocalyx and its function, cellular junctions. The cell surface: structure, composition and function of the cell membrane, transport mechanisms, differentiation of the cell surface (
The cytoplasm: 20 hours
- the cytoskeleton: microtubules, microfilaments and intermediate filaments,
-The smooth and rough endoplasmic reticulum, the Golgi apparatus and cellular secretion, lysosomes, phagocytosis and endocytosis
-the peroxisomes, mitochondria
The nucleus: 8 hours
the nuclear envelope. Chromatin. Chromosomes: structural models, techniques for the study of chromosomes The nucleolus and biogenesis of ribosomes structure and ultrastructure of ribosomes Mitosis Meiosis Apoptosis;
Epithelial tissue: 8 hours
exocrine glands, endocrine glands and their function
Connective tissue: 12 hours
The connective tissues, connective tissue of support: cartilage, bone, ossification.
blood: plasma, serum, erythrocytes, leukocytes, platelets.).
Muscle tissue: 4 hours
smooth, skeletal and cardiac muscle tissue.
Nervous tissue: 4 hours
The course includes 72 hours of lectures, and practical laboratory activities.
Preparation of the histological slides: slicing with the microtome, distension of the slices on the slide.
Use of the optical microscope
Observation of histological preparations: epithelial tissue, proper connective tissue, bone tissue, cartilage tissue, blood, muscle tissue, nervous tissue.
Lectures in pdf format provided by the teacher during the course, available on the link e-learning of Science and Technology Department.
BIOLOGIA- cellula e tessuti (ED. Ermes). Colombo -Olmo
Texts to be refer for more details.
Alberts et al.: Biologia Molecolare della cellula –Essenziale . Ed. Zanichelli.
Wolfe: Biologia molecolare e cellulare. (Ed. Edises.
Fawcett: Trattato di Istologia (12°ed.)Ed McGraw – Hill.
Wether: Istologia – Casa Editrice Ambrosiana
Kerr : Istologia funzionale. Casa Editrice Ambrosiana.
The objective of the exam is to check the level of achievement of the above-mentioned training objectives. The exams consists of two tests elapsed written, a final oral examination and recognition of histological preparations under the microscope and / or on histology atlases.