Università degli Studi di Napoli "Parthenope"

Teaching schedule

Academic year: 
2021/2022
Belonging course: 
Course of Bachelor's Degree Programme on BIOLOGICAL SCIENCES
Disciplinary sector: 
MOLECULAR BIOLOGY (BIO/11)
Language: 
Italian
Credits: 
6
Year of study: 
2
Teachers: 
CICCODICOLA Alfredo
Cycle: 
Second semester
Hours of front activity: 
48

Language

ITALIAN/ENGLISH

Course description

The training objectives of the course in Molecular Biology plan to provide the students the ability to understand the molecular structure of nucleic acids, the structural organization of the gene and the molecular mechanisms that regulate its function and replication. The applications of some of the fundamental technologies of recombinant DNA are also presented, useful as a tool for basic and applied research.
Knowledge and understanding: The student must demonstrate knowledge, understanding and reworking the molecular aspects studied during the course of Molecular Biology in order to transform the knowledge learned in a more complex reflection and elaboration.
Ability to apply knowledge and understanding: The student must demonstrate the ability to use the concepts acquired even in areas other than those in which the knowledge learned of Molecular Biology are traditionally used.
Autonomy of judgments: Students must be able to autonomously explore what they have learned about the aspects of Molecular Biology, in order to use the acquired knowledge as a starting point that allows them to achieve further results, characterized by a growing maturity and from an ever wider autonomy of judgment.
Communication skills: The student must have the ability, in a clear and complete way, to demonstrate the contents of the topics related to the course of Molecular Biology using correctly the scientific language; communication must also be understandable to those who do not have specific preparation on the subject.
Learning skills: Attendance is a teaching aid with central importance; however, the student must be able to update himself continuously, through the consultation of texts and publications (also in English) in order to acquire the ability to deepen the topics in the field of Molecular Biology.

Prerequisites

No prerequisites are required for the course, even if the student is advised to have passed the Biochemistry exam. Also, it notes that the examination of Molecular Biology is a prerequisite for the examination of Genetics.

Syllabus

Struttura e funzione degli acidi nucleici: Nucleotidi; Derivati nucleotidici; Legame fosfodiesterico; Studi di Chargaff e Franklin-Wilkins; Modello di Watson e Crick; Conformazioni; Flessibilità; Forze che stabilizzano.
Struttura, funzione del nucleosoma e della cromatina; Interazione dinamica del DNA-nucleosoma; Complessi di rimodellamento; Modifiche istoniche; Bromo- e cromo-domini; Assemblaggio e diassemblaggio.
Replicazione del DNA: Inizio, Allungamento, Frammenti di Okazaki e Terminazione; DNA polimerasi; Replicazione circolare, a cerchio rotante e dei telomeri.
Trascrizione: RNA polimerasi; Promotori; Inizio e Allungamento; Terminatori Rho-dipendenti e -indipendenti; Complesso del mediatore; Trasduzione del segnale.
Maturazione del RNA: Capping al 5’ e poliadenilazione; Splicing e Spliceosoma; Splicing alternativo; Cooperazione dei siti di splicing e regolazione; Editing del RNA; Deaminazioni delle basi; RNA ribosomiale e Trascrizione; Assemblaggio e struttura dei ribosomi. RNA transfer; Aminoaciliazione; Codice genetico; Vacillamento, espansione ed evoluzione del codice genetico.
Sintesi proteica: Inizio della traduzione; Formazione del legame peptidico; Allungamento del polipeptide; Termine della traduzione; Modifiche post-traduzionali; Smistamento delle proteine.
Regolazione dell’espressione genica nei batteri: Geni inducibili; Il lattosio come fonte di carbonio; Modello di Jacob e Monod; Controllo positivo dell’operone lac; Operoni reprimibili; Operone triptofano; Modello dell’attenuazione
ncRNA: snoRNA; Processamento del rRNA; snRNA; Riboswitch; Biogenesi dei miRNA; piRNA; RNA antisenso e interference; lncRNA.
Paradosso del valore C; Complessità, Composizione e Organizzazione del genoma; Isocore; Cinetiche di riassociazione; Sequenze ripetute; DNA satellite; Duplicazioni segmentali; Retrotrasposoni; SINE e LINE; Mini- e Micro-satelliti; Pseudogeni; Famiglie geniche.
Mutazioni geniche, Puntiformi e nei siti di splicing; Mutazioni spontanee e indotte; Analoghi delle basi, Agenti che modificano le basi e intercalanti; Riparo.
Tecniche Molecolari: Endonucleasi, Ligasi, fosfatasi e chinasi; PCR; Taq-Polimerasi; Varianti; Real Time; Sequenziamento del DNA; Metodi di Maxam-Gilbert, Sanger e NGS; Estrazione del DNA; Elettroforesi; Southern e Northern blot; Organismi modello; Ricerca delle mutazioni.

The course of Molecular Biology provides information to the student to understand the molecular structure of nucleic acids, the structural organization of the gene and the molecular mechanisms that regulate its function and replication. The applications of some of the fundamental technologies of recombinant DNA are also presented, useful as a tool for basic and applied research.
The main topics of the course are provided as Blended lessons in English on the e-learning site (http://e-scienzeetecnologie.uniparthenope.it).

Teaching Methods

The course of Molecular Biology is divided into 48 hours of lectures. Furthermore, on the e-learning site there are some essential lessons in Blended format, delivered in English.

Textbooks

The slides of each lesson of the course, and other teaching material will be made available during the course in .pdf format and uploaded to the e-learning site (http://e-scienzeetecnologie.uniparthenope.it).
The reference texts in Italian:
• Biologia molecolare del gene - Watson, Baker, Bell, Gann, Levine, Losick - Zanichelli – 7a - 2015
• Biologia Molecolare - G. Capranico, E. Martegani, G. Musci, G. Raugei, T. Russo, N. Zambrano, V. Zappavigna- EdiSES - I/2016
The reference texts in English:
• Molecular Biology of the Gene, 7th Edition - Watson, Baker, Bell, Gann, Levine, Losick - Pearson - 2014
Specific topics can be explored through scientific articles indicated during the course and uploaded to the e-learning site.

Learning assessment

The objective of the exam is to verify the level of achievement of the previously indicated training objectives.
The exam involves assessing the student's ability to connect and compare different aspects covered during the course.
The exam consists of an oral test during which you will be asked to trace the structure of the nucleotides that make up the nucleic acids.
The evaluation of the test takes into account the efficiency of the methods used, the completeness and accuracy of the answers, as well as the clarity in the presentation.
The exam for foreign students can be taken in English.

More information

The teaching material is accessible in e-learning mode at the link http://e-scienzeetecnologie.uniparthenope.it
The reception time is Tuesday and Thursday from 10:00 to 13:00. The meeting with the teacher must be agreed by email sent from the institutional email address and can take place both in person and remotely via the Teams platform.